Featured article:
Integrative modules for efficient genome engineering in yeast
Triana Amen and Daniel Kaganovich
We present a set of vectors containing integrative modules for efficient genome integration into the commonly used selection marker loci of the yeast Saccharomyces cerevisiae. A fragment for genome integration is generated via PCR with a unique set of short primers and integrated into HIS3, URA3, ADE2, and TRP1 loci. The desired level of expression can be achieved by using constitutive (TEF1p, GPD1p), inducible (CUP1p, GAL1/10p), and daughter-specific (DSE4p) promoters available in the modules. The reduced size of the integrative module compared to conventional integrative plasmids allows efficient integration of multiple fragments. We demonstrate the efficiency of this tool by simultaneously tagging markers of the nucleus, vacuole, actin, and peroxisomes with genomically integrated fluorophores. Improved integration of our new pDK plasmid series allows stable introduction of several genes and can be used for multi-color…
About us
News
Meet the editorial board
Most viewed last month
- Protein aggregation triggers a declining libido in elder yeasts that still have a lust for life
- Integrative modules for efficient genome engineering in yeast
- Electron microscopy for ultrastructural analysis and protein localization in Saccharomyces cerevisiae
- Formyl-methionine as a degradation signal at the N-termini of bacterial proteins
- Metabolites in aging and autophagy
- Understanding structure, function, and mutations in the mitochondrial ATP synthase
- Sulfur transfer and activation by ubiquitin-like modifier system Uba4•Urm1 link protein urmylation and tRNA thiolation in yeast
- Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling
- Recent Insights into the HIV/AIDS Pandemic
- Translate to divide: сontrol of the cell cycle by protein synthesis
Publish with MIC
Social media
Newsfeeds
