Figure 3: Pim1-GFP localization changes with age. Changes in protein localization were monitored in MEP cells expressing Pim1-GFP (a, b). Nup49-mCherry was used as marker of the nuclear periphery. Upper and lower images corresponding to the same division, but 20-40 min apart, are shown to detect possible cell cycle-dependent changes in localization. Localization of Pim1 in daughter cells (b) is shown. Stacks of 4 sections (0.5 µm spacing) acquired at different time points were deconvolved (maximal projection is shown). Bright-field images were used to draw cell boundaries and count the number of divisions for the mother cells (age indicated with numbers). Representative images are shown. All five cells analysed showed the same phenotype depicted and described in the text. Scale bar, 2 µm.