FIGURE 3: The Ydj1 interaction network is modulated by heat shock. AP-MS of 2xFLAG tagged YDJ1 was performed from FLAG-YDJ1/ydj1Δ cells grown at 30^°C or subjected to a 15 minute 30^°C-42^°C heat shock. Statistically significant Ydj1 interaction partners are shown as a dot plot in which the node color corresponds to the absolute number of spectral count identified for prey proteins, the node size corresponds to the relative abundance between the untreated or heat shock sam-ple, and the node edge represents the SAINT FDR rate at which a given prey protein was observed.