FIGURE 2: PLSDA of WT and purH mutant metabolomes. Untargeted metabolomics was performed on wild-type and purH mutant of S. enterica grown in glucose minimal medium supplemented with adenine and thiamine. Partial Least Square Discriminate Analysis (PLSDA) was used as a multivariate analysis tool to provide insight into the unique metabolic fingerprint of the purH mutant. Each dot represents one experimental replicate; WT is in cyan, the purH mutant is in peach. Confidence intervals are shown by the colored ellipses.