FIGURE 3: The metabolome of a purH mutant is significantly altered. Untargeted metabolomics was performed on wild-type and purH mutant of S. enterica grown in glu-cose minimal medium supplemented with adenine and thiamine. Relative concentrations of metabolites were determined by calculating the ratio of the average MS ion intensities (peak areas) in wild-type and purH strains (purH/WT). Here the log transformed fold changes (Log2) and p-values (-Log10) were plotted against each other, distributing the metabolite pool decreases and increases to the left and right of the x=0 point, respectively. The horizontal line (y=1.3) indicates the threshold above which p-values were considered statistically significant (<0.05). These data are derived from one representative experiment (Table S2).