FIGURE 4: AICAR perturbs various areas of metabolism. Untargeted metabolomics was performed on wild-type and purH mutant of S. enterica grown in glucose minimal medium supplemented with adenine and thiamine. Relative concentrations of metabolites were determined by calculating the ratio of the average MS ion intensities (peak areas) in wild-type and purH strains (purH/WT). Here the log transformed fold changes (Log2) of metabo-lites with statistically significant differences were plotted to distribute the decreases and increases (below and above) the y-axis. Each point along the x-axis represents a metabolite. Each of 3 experimental replicates are differentiated by color. Specific metabolites, fold changes and p-values are provided in Table S3.