TABLE 2. Metabolomics captures known metabolic changes of a purH mutant.

Untargeted metabolomics was performed on WT and ΔpurH mutant of S. enterica grown in glucose minimal medium supplemented with adenine and thiamine. Relative concentrations of 3 key metabolites whose physiological changes have been previously described were extracted.

^a Experimental replicates consisted of 3 biological replicates of each strain.

^b Fold changes were the ratio of the average MS ion intensities (peak areas) in WT and ΔpurH strains (ΔpurH/WT).

^c p-values indicative of statistically significant differences are noted as follows <0.05 (**), <0.01 (***).