FIGURE 2: LRV1 promotes the transcription of the inflammasome components, caspase-1, IL-1β, NLRP3 and AIM2. BMDMs from C57BL/6 mice were infected with LgyLRV1+ or LgyLRV1- parasites or stimulated with a synthetic TLR3 ligand, poly(I:C). After 6 hours and 12 hours, mRNA was quantified by qRT-PCR. (A) The common inflammasome components ASC, Caspase-1 and IL-1β and the inflammasome-forming members of the (B) NLRC family, (C) NLRP family and (D) a non-NLR inflammasome-forming molecule, AIM2 were measured. Levels are representative of both 6-hour and 12-hour time points. Representative results are shown from at least three independent experiments, using two technical and two biological replicates, and showed as mean ± SEM. Non-parametric Student’s t-test is shown. *p < 0.05, **p < 0.01, ***p < 0.001. A cut off value of 2-fold was used as a limit of significance and is indicated as a dotted line.