Back to article: Valine biosynthesis in Saccharomyces cerevisiae is regulated by the mitochondrial branched-chain amino acid aminotransferase Bat1
FIGURE 1: Effects of Bat1 and Bat2 on the growth and BCAAs contents of yeast cells. (A) Growth phenotypes of S. cerevisiae wild-type (BY4741), ∆bat1, and ∆bat2 cells harboring pRS415/pRS416 cultured on SD+His/Met, SD+His/Met/Val or SD+His/Met/Val//Ile/Leu medium. (B) Intracellular BCAAs contents of S. cerevisiae wild-type (BY4741), ∆bat1, and ∆bat2 cells harboring pRS415/pRS416 cultured in SD+His/Met medium. White, striped, and dark gray bars represent intracellular valine, leucine, and isoleucine contents, respectively. The values are the means and standard deviations of three independent experiments. Statistically significant differences were determined by Student’s t test (a,bp <0.01).