FIGURE 3: Effects of mitochondrial BCAT on the growth and BCAAs contents of yeast cells. (A) Growth phenotype of S. cerevisiae wild-type (BY4741) (open square), ∆bat1 (open circle), ∆bat2 (closed circle), and ∆bat1∆bat2 cells harboring pRS416-Bat1-MTS/pRS415 (open triangle) or pRS415-Bat2+MTS/pRS416 (closed triangle) in SD+His/Met liquid medium. The values are the means and standard deviations of three independent experiments. (B) Intracellular BCAAs contents in S. cerevisiae wild-type (BY4741), ∆bat1, ∆bat2, and ∆bat1∆bat2 cells harborin pRS416-Bat1-MTS/pRS415 or pRS415-Bat2+MTS/pRS416 cultured in SD+His/Met medium. White, striped, and dark gray bars represent intracellular valine, leucine, and isoleucine contents, respectively. Data shown are the average and the standard deviation of three independent experiments. The values are the means and standard deviations of three independent experiments. Statistically significant differences were determined by Student’s t test (^a,bp <0.05). (C) Growth phenotype of S. cerevisiae ∆bat1∆bat2 cells harboring pRS416-K219A-Bat1 and pRS415-K202A-Bat2 cultured on SD+His/Met, SD+His/Met/Val/Ile, and SD+His/Met/Val/Ile/Leu medium, respectively.