Back to article: Snf1 cooperates with the CWI MAPK pathway to mediate the degradation of Med13 following oxidative stress


FIGURE 3: Snf1, Sak1 and at least one β subunit are required for degradation of Med13 following H2O2 stress. (A) Wild-type (RSY10), snf1∆ (RSY2080), sak1∆ (YPDahl17) and gal83∆ sip1∆ sip2∆ (MSY557) cells harboring full length Med13-HA (pKC801) were treated with 0.4 mM H2O2 for the timepoints indicated and Med13-HA levels analyzed by Western blot. Tub1 levels were used as a loading control. (B) snf1∆ cells harboring Med13-HA (pKC803) and either wild- type Snf1 (JG1193), a vector control (pRS316) or snf1K84R (JG1338) were treated and analyzed as described in A. (C and D) Degradation kinetics of the Med13-HA shown in A and B. Values represent averages ± SD from a total of at least two Western blots from independent experiments.

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