Back to article: Snf1 cooperates with the CWI MAPK pathway to mediate the degradation of Med13 following oxidative stress
FIGURE 4: Snf1 activation does not mediate Med13 degradation. (A) Mid-log -type cells (RSY10) growing in 2% glucose (T=0) were switched to media containing 0.05% glucose. Snf1 phosphorylation and Snf1 itself were detected as described in materials and methods for the timepoint indicate. (B) Wild-type cells harboring Med13-HA (pKC801) were switched to media containing 0.05% glucose. Med13-HA levels were detected by Western blot analysis for the timepoints indicated. Tub1 was used as a loading control. (C) As in (A) except that Snf1 phosphorylation was monitored after 0.4 mM H2O2 treatment in the strains indicated.