FIGURE 3: PolyP and VTC4 fluctuation during promastigote growth of L. major and L. mexicana. L. major (A) and L. mexicana (C) promastigote cell concentrations were counted in independent cultures. (B, D) PolyP was extracted from cell lines, digested with polyphosphatase and P_i were quantified colorimetrically using malachite green. P_i concentrations represented polyP content of 3 x 10⁷ cells for L. major (B) and 8 x 10⁶ cells for L. mexicana (D). Results of a pool of minimum 3 independent experiments were expressed as mean ± SD. (E) Western blot analysis of 20 µg of L. major promastigotes pellet protein using the anti-cd-LmjVTC4 antibody and anti-α-tubulin as loading control.