FIGURE 4: In vitro proliferation of VTC4 knockout L. major parasites. L. major WT or vtc4^– promastigotes were diluted at a concentration of 5 x 10⁵ parasites per ml and cultured in complete medium. Cell concentrations were counted in independent cultures. Results of a pool of 4 independent experiments were expressed as mean ± SD. Statistical significance was assessed by Student’s t-test; * p < 0.05, *** p < 0.01, ns: non-significant.