FIGURE 1: Effects on growth and culture density of miltefosine, staurosporine, PBS and of the absence of FCS. (A-B) Cell concentration after culture for 24 h in different conditions (A) or for 4 h in PBS (B). Mean ± SD from a minimum of three independent experiments. Unpaired t-test between the condition of interest at 24 h and the control condition at 24 h. (C) Optical density of cells cultivated in different conditions after addition of MTT. A minimum of three independent experiments were carried out. Unpaired t-test between the condition of interest at 24 h or 4 h and the same condition at 0h: ns not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.