FIGURE 2: Growth assay of S. cerevisiae tps1Δ mutant transformed with TPS1 from different organisms on solid media. The Sctps1Δ strain transformed with YcpLac33 plasmid bearing TPS1 homologue was cultivated on YN trehalose 1 % till 5 OD600 units. 10 μl of serial dilution from this preculture was spot-ted on YN agar plate containing glucose or galactose at 2% (w/v). Growth was scored after 2 days at 30°C.