FIGURE 5: Heat map representation of the change in T6P (A), H6P (B) and FBP (C) concentration in S. cerevisiae tps1 mutant expressing TPS1 from different organisms in re-sponse to a glucose pulse. The Sctps1 strain transformed with YCpL33 plasmid bearing TPS1 homologue was cultivated on YN Trehalose 1% till 5 OD600 units. Then, glucose (10 g/L final concentra-tion) was added and samples (5 ml) at different times were rapidly collected by filtration for intracellular metabolite ex-traction and quantification. The data used for the heat map were the mean values of three independent experiments. Abbreviations: H6P mean hexose-6-Phosphate which is the sum of Glc6P and Fru6P; FBP means Fructose 1, 6-bisphosphate.