FIGURE 8: Expression level of FBP1 (A) and PCK1 (B) in S. cerevisiae tps1 mutant complemented with TPS1 from different organisms. The Sctps1 strains transformed with YCpL33 plasmid and with TPS1 homologues were cultivated on YN containing etha-nol 2% until 5 units OD600. At this time, glucose (10 g/L final) was added and samples (15 OD units) were taken before and 1 h after glucose addition. The transcript levels of FBP1 and PCK1 was quantified by RT-qPCR using TAF10 and KRE11 as reference genes for normalization. Results shown are the mean of two.