FIGURE 4: MutS dynamics in B. subtilis cells. (A) Effective diffusion coefficients, D, of MutS-PAmCherry as a function of separation distance from the nearest replisome. Error bars indicate 95% confidence interval. WT+ indicates cells that have been treated with the 2-AP mutagen. (B) Cross-correlation between the separation between MutS and the center of DnaX cluster and the instantaneous speed of MutS. (C) Cumulative probability distribution of the time, t, that MutS (red “S”) spends within the same replisome region (blue “R”). Reproduced from Liao et al. PNAS 2015 [41].

41. Liao Y, Schroeder JW, Gao B, Simmons LA, Biteen JS (2015). Single-molecule motions and interactions in live cells reveal target search dynamics in mismatch repair. Proc Natl Acad Sci U S A 112(50): E6898-E6906. 10.1073/pnas.1507386112