Back to article: Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes
FIGURE 11: Gel-based endonuclease assay using a fluorescent substrate. Time course analysis for 1, 3, 5, 7, 10, and 15 minutes with 100 nM of the Cy3/Cy5 3′-flap substrate and 5 nM human MUS81-EME1 visualized on a 10% native acrylamide gel with Cy3 and Cy5 filters.