FIGURE 5: The deletion of TOR1 or SCH9 decreases ROS production, attenuates catalase A deficiency and diminishes apoptotic cell death in isc1Δ cells.
(A) S. cerevisiae BY4741, isc1Δ, tor1Δ and isc1Δtor1Δ, sch9Δ and isc1Δsch9Δ cells were grown to early stationary phase (day 1 in the CLS assay), stained with the dihydroethidium (DHE) for 10 min and analyzed by flow cytometry. Values are mean ± SD of at least three independent experiments. ***p<0.001; **p<0.01.
(B) Yeast cells were grown to the PDS phase and catalase activity was detected in situ after non-denaturing polyacrylamide gel electrophoresis, using the H2O2/peroxidase system, as described in Materials and Methods. A representative experiment out of three is shown.
(C) BY4741, isc1Δ, tor1Δ and isc1Δtor1Δ cells were double stained with DiOC6(3) and PI (propidium iodide) and analyzed by flow cytometry, as described in Materials and Methods. Representative histograms are shown.