FIGURE 2: Schematic overview of the novel peroxisome biogenesis pathway.

The upper pink part represents the presence of pre-peroxisomal vesicles, containing Pex13 and Pex14, in yeast PEX3 deletion cells. These vesicles may be autonomous and proliferate by fission or form from the ER in a Pex3/Pex19 independent manner. Upon reintroduction of Pex3 (lower part in green), the protein most likely directly travels to the pre-peroxisomal vesicles or alternatively, reaches these via ER derived vesicles. After incorporation of Pex3 in the pre-peroxisomal vesicles, the other PMPs insert into the vesicles via the Pex3/Pex19 dependent docking machinery (see also Fig. 1A).