FIGURE 2: The IGP family are developmentally regulated ER proteins.
(C) Digestion of IGP48 with PNGase F or Endo H, or treatment with tunicamycin results in a large molecular weight shift. IGP48 was detected in fractionated lysates using anti-HA antibody.
(D) Turnover of IGP48 and IGP40. Quantification of anti-HA reactivity in lysates of cells expressing IGP48-HA and IGP40-HA following inhibition of protein synthesis with cycloheximide. Error bars represent the standard deviation and values were normalised against a loading control, BiP (n = 3).
Go to figure 2A-B.
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