FIGURE 4: Localisation of IGP sorting signals.
(A) Immunofluorescence demonstrating the locations of IGP constructs in BSF trypanosomes. HA-tagged constructs were detected in permeabilised cells with anti-HA antibody (red). The ER was stained with anti-TbBiP (green) and DNA stained with DAPI (blue). BiPN constructs, in which the lumenal/ecto-domain is replaced by the BiP ATPase domain, no longer co-localise with TbBiP and so are not retained within the ER.
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