FIGURE 4: Losartan prevents Cu-induced apoptosis in HepG2 cells.

(a) Losartan increases HepG2 cell viability in presence of Cu. HepG2 cells were treated with Cu (0.75 mM – 1.25 mM) in presence of control (1% DMSO, black bars), 25 µM Candesartan Cilexetil (white bars) or 100 µM Losartan (grey bars). Following 48 h of incubation, cell viability was determined by MTT viability staining, and expressed as compared to cells receiving no Cu. Biological repeat is four. (***P < 0.001; ANOVA test using Tukey correction).

(b) Losartan prevents Cu-induced apoptosis in HepG2 cells. HepG2 cells were treated for 1.5 mM Cu in absence (control) or presence of 100 µM Losartan for 24 h. Subsequently, cells were stained with FLUOS-labeled Annexin V and samples were analyzed by flow cytometry. Biological repeats is three. (**P < 0.01; Student t-test).