FIGURE 4: Lack of Mpc1 results in low levels of TCA cycle intermediates and enhanced malic enzyme activity.
(A) Scheme of the TCA cycle and of the glyoxylate shunt. Ach1, acetyl-CoA hydrolase 1; Acs, acetyl-CoA synthase; Adh, alcohol dehydrogenase; Ald, aldehyde dehydrogenase; Icl1, isocitrate lyase 1; Mae1, malic enzyme; Pck1,phosphoenolpyruvate carboxykinase 1; Sfc1, succinate-fumarate carrier.
Wt and mpc1Δ cells were grown as in Figure 2 and at the indicated time points the concentrations of citrate (B), succinate (C) and malate (D) were measured.
The bar chart of malic enzyme activity (E) is also reported.
Exp, exponential growth phase. Day 0, diauxic shift. Data refer to mean values determined in three independent experiments. SD is indicated.* P ≤ 0.05 and ** P ≤ 0.01.