FIGURE 5: Analysis of total translation in yeast cells.

(A) Total translation in yeast cells expressing PAP and AtBI-1.

(B) Total translation in yeast cells expressing PAP and AtBI-1ΔC.

Yeast cells were grown in SD-L-U-Met and 2% glucose overnight then switched to 2% galactose containing media to induce the expression. At time 0, [³⁵S] methionine was added to cells growing on galactose which expressed PAP or AtBI-1 and incorporation of [³⁵S] methionine was determined at the indicated times. Each point was repeated in duplicate. The results represent three independent experiments. VC – vector control. Columns are statistically different according to ANOVA (P < 0.001) followed by a post-hoc Fisher’s Least Significant Difference (LSD) test.