FIGURE 5. Spermine does not affect the affinity of antizyme to ODC.

Co-pull down of Oaz1 and ODC in the presence or absence of 1 mM spermine (spm). Extracts from E. coli cells expressing the indicated tagged proteins were subjected to GST-pull down and subsequent quantitative anti-Flag western blotting for ODC-Flag detection and anti-GST for GST-Oaz1 detection. ODC-Flag signals after elution were normalized to GST-Oaz1 signals providing ODC-Flag values obtained in the absence of spm, the mean of which was set to 100%. Values obtained in the presence of spm are given in % of those obtained in its absence. Error bars, s.d.; n = 3.