FIGURE 4: Haploid cells carrying both mim3-1 and nam3-1 suppressors are respiratory deficient.
Fresh cultures of different strains are streaked on YPGA (glucose) and N3 (glycerol) plates and incubated three days at 28°C. All of these strains are isogenic: the nucleus is from JC8 and the mitochondria are from 777-3A. The nuclear genotype is given in brackets (with the nuclear one at the left and the mitochondrial at the right) with N+: NAM3+, n1: nam3-1; n2: nam3-2, n3: nam3-3; m: mim3-1; M: M3041.