Figure 6 The MAPKKKs Ste11 and Bck1 jointly transmit the oxidative stress signal to cyclin C

FIGURE 6: RHO1^G19V promotes cyclin C-dependent mitochondrial fission and programmed cell death in the absence of stress.

(A) Mitochondrial morphology was followed by fluorescence microscopy in wild type (RSY10) and cnc1∆ (RSY391) cultures expressing mt-dsRed and either a vector control or RHO1^G19V. Representative images (0.2 µm slices) of each cell type are shown. The arrows indicate cells exhibiting fission in the cnc1∆ mutant.

(B) Quantitation of three independent isolates exhibiting reticular or fragmented mitochondria were determined from ≥ 600 cells per culture (mean ± s.e.m.) p values for then indicated data sets are shown.

(C) Mitochondrial morphology was followed by fluorescence microscopy in bck1∆ (RSY1050) and bck1∆ cnc1∆ (RSY1052) cultures expressing mt-dsRed and either a vector control or RHO1^G19V

(D) Quantitation of three independent isolates exhibiting reticular or fragmented mitochondria were determined from ≥ 600 cells per culture.