FIGURE 2: Utilization of carbohydrate and lipid storage compounds in aerobic and anaerobic stationary-phase cultures.

Cellular contents of glycogen (A) and trehalose (B) are shown for the SP of glucose-grown batch cultures of S. cerevisiae, grown under aerobic (open symbols, ○) or anaerobic (closed symbols, ●) conditions. Panel (C) shows cellular contents of total fatty acids (closed circles, ●), palmitoleic acid (open circles, ○) and palmitic acid (closed squares, ∎) in aerobic SP cultures. Time point zero indicates the onset of SP, which corresponds to exogenous glucose exhaustion in anaerobic cultures and exhaustion of all exogenous carbon sources including ethanol and organic acids in aerobic cultures. Average and SD or SEM are shown, calculated from either quadruplicate cultures (glycogen and trehalose) or duplicate cultures (fatty acids), respectively.