FIGURE 2: HPLC analysis examining the relationship between mcm⁵s²U₃₄ and t⁶A₃₇.

(A) Analysis of mutations in mcm⁵s²U synthesis with detection at 313 nm specific for thio-moieties. Black line = BY4741; Orange = trm9∆; Pink = elp3∆; Blue = ncs2∆; Green = ncs6∆.

(B) Analysis of mcm⁵s²U in mutants for t⁶A synthesis for with detection at 313 nm. Black = BY4741; Green = tcs2∆; Blue = tcs3∆; Brown = tcs5∆; Pink = tcs6∆; Orange = tcs7∆; Teal = tcs8∆.

(C) Analysis for t⁶A in mutants of mcm⁵s²U synthesis with detection at 254 nm. The color scheme is the same as part A, with the t⁶A standard in red.

(D) Analysis for t⁶A in mutants of t⁶A synthesis with detection at 254 nm. Color scheme is the same as part B, with the t⁶A standard in red.