FIGURE 3: Seven drugs suppress Aβ42-RF oligomerization in yeast in a dose-dependent manner.

SDS-resistant Aβ42-RF oligomers were detected by immunoblot analysis (upper). The assay strain expressing Aβ42-RF was grown in complex medium in the presence of each compound at the indicated concentrations. Equal amounts of lysate proteins were treated with 1% SDS for 7 mins at room temperature and analyzed by SDS PAGE followed by immunoblotting with anti-Sup35 RF antibodies. Aβ42-RF cells grown in DMSO (0) were used as controls. PGK, yeast 3-Phosphoglycerate Kinase, detected with anti PGK antibodies was an internal control to show the effect of the drugs on protein synthesis in general. Immunoblot signals for Aβ42-RF monomers and oligomers were quantified and converted into % inhibition of oligomer formation from the ratios of oligomers to monomers compared to DMSO controls (0 means no inhibition) (lower). Error bars represent standard deviation from 3 independent immunoblots except for ifenprodil tartrate which shows data from 1 of 2 immunoblots that showed no drug effects. The asterisks show significant levels of **P < 0.01 or *P < 0.05 according to Student’s t test.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this. Please refer to our "privacy statement" and our "terms of use" for further information.

Close