FIGURE 5: The effect of drugs that inhibit Aβ₄₂-RF from forming oligomers on toxicity caused by overexpression of HDEL-Aβ₄₂ in yeast.

Effects of 7 drugs on suppression of HDEL-Aβ₄₂ toxicity and general cell growth are shown. Yeast cells carrying either HDEL-Aβ₄₂ (HDEL-Aβ₄₂ strain) or an empty vector (control) grown overnight in non-inducing plasmid selective media (2% raffinose, SR-Leu) were normalized, diluted to 1 x 10⁵ cells/100 µl of media in each of 96 wells and further grown for 3 days in 2% galactose inducing media (SRGAL-Leu) or 2% raffinose noninducing media (SR-Leu) in the presence of each drug at the indicated concentrations. Growth was measured as OD₆₀₀. Error bars show the standard deviation from three trials. The asterisks show increased relative growth with drug treatment compared to DMSO control (black bar in left panel) at significance levels of **P < 0.01 or *P < 0.05 according to Student’s t test.