FIGURE 5: The effect of drugs that inhibit Aβ42-RF from forming oligomers on toxicity caused by overexpression of HDEL-Aβ42 in yeast.

Effects of 7 drugs on suppression of HDEL-Aβ42 toxicity and general cell growth are shown. Yeast cells carrying either HDEL-Aβ42 (HDEL-Aβ42 strain) or an empty vector (control) grown overnight in non-inducing plasmid selective media (2% raffinose, SR-Leu) were normalized, diluted to 1 x 105 cells/100 µl of media in each of 96 wells and further grown for 3 days in 2% galactose inducing media (SRGAL-Leu) or 2% raffinose noninducing media (SR-Leu) in the presence of each drug at the indicated concentrations. Growth was measured as OD600. Error bars show the standard deviation from three trials. The asterisks show increased relative growth with drug treatment compared to DMSO control (black bar in left panel) at significance levels of **P < 0.01 or *P < 0.05 according to Student’s t test.

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