FIGURE 6: The meiotic recombination checkpoint response is impaired in the absence of Sir2 or Sas2.

(A) Time course of meiotic nuclear divisions; the percentage of cells containing two or more nuclei is represented.

(B) Dityrosine fluorescence, as a visual indicator of sporulation, and sporulation efficiency, quantified by microscopic examination of at least 300 cells, were examined after 3 days of sporulation on plates.

(C) Western blot analysis of the indicated proteins during meiosis. Strains are DP421 (wild type), DP422 (zip1), DP1401 (zip1 sir2) and DP1410 (zip1 sas2).