FIGURE 6: Cell survival and colony formation upon MMS treatment.

(A and B) FACS analysis of PI staining following 12 h of MMS treatment. Bars represent the PI-positive percentage fractions of cells as measured using FACS. Results feature three biologically independent replicates with three technical replicates each. Error bars show the standard deviation. Statistical significance was determined using unpaired student t-test assuming equal variance. (*: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001). The brackets over the wt Vector and fbp1∆ Vector strains the control vector strains show the significance comparing treated and non-treated of the same strain. While significance stars over the mutants represent significance when comparing the results form these strains with those of the control vector.

(C and D) colony forming on SD-Ura agar following 12 h of MMS treatment in liquid SD-Ura. Results are from 3 independent experiments. Error bars show the standard deviation. Asterisks indicate statistically significant differences compared to the control vector strains. Significance was assessed using student t-test (*: p < 0.05).