INTRODUCTION

Progesterone is a sterol-derived hormone that is crucial for female reproductive capacity and plays major regulatory roles in the monthly menstrual cycle and upon conception as well as during pregnancy and embryogenesis. In addition, it also serves as a neurosteroid, thus playing an important role in brain function in both sexes [1]. For instance, progesterone inhibits the neuronal nicotinic acetylcholine receptor and stimulates the synthesis of myelin proteins [1]. Of note, progesterone has been linked to the gender-specific risk and outcome of brain injuries that is more favorable for females [2]. Interestingly, preclinical data strongly suggest that (high doses of) progesterone may positively affect recovery from traumatic brain injury (TBI) in model organisms [3][4][5][6][7], if administered before or shortly after TBI. Two clinical studies could confirm a neuroprotective effect of progesterone when administered shortly after TBI [8][9], while some more recent clinical data seem to disprove this hypothesis [10][11][12]. Therefore, it remains an open question if progesterone affects the recovery and survival after TBI in humans and to which extent it promotes cellular restauration.

–

In order to investigate the cytoprotective potential of progesterone, we took advantage of Saccharomyces cerevisiae, knowing that this organism has repeatedly been shown to be suitable for mechanistic studies of programmed cell death (PCD) [13][14][15][16][17][18][19]. Yeast is especially useful as a model to study neuroprotection at the cellular level [20][21][22][23][24][25][26][27]. Here, we describe the positive impact of progesterone on several parameters of cellular physiology. Importantly, our results also suggest a possible receptor-independent mechanism for these effects, since deletion of DAP1 – a heme-binding protein related to the mammalian membrane progesterone receptor – did not alter susceptibility towards progesterone treatment. Altogether, we reveal that progesterone exerts potent cytoprotective effects in yeast.

RESULTS

Progesterone increases stress tolerance

Traumatic brain injury is connected to elevated PCD and ROS accumulation in the brain tissue [28][29]. Therefore, we tested if progesterone would render yeast cells less susceptible towards different stressors that are connected to an increase in ROS production. Upon addition of progesterone, wild type yeast cultures treated with H₂O₂ or acetate, which are both well-known PCD inducers in yeast [14][30][31][32][33][34], showed reduced ROS accumulation as measured by the ROS-driven conversion of dihydroethidium (DHE) to fluorescent ethidium (Figure 1A and B). Furthermore, under physiological culture conditions, in the absence of PCD inducers, progesterone significantly reduced ROS levels as compared to the untreated control (Figure 2A). Altogether, progesterone dampens ROS production in yeast, both in normal culture conditions and in the presence of external stress factors.

–

FIGURE 1: Progesterone treatment increases resistance of wildtype yeast to external stressors. ROS accumulation in yeast cells treated with progesterone (10 µg/ml) or left untreated as shown by the DHE to ethidium turnover rate upon hydrogen peroxide (A) or acetate (B) challenge during logarithmic phase. All data represent mean values (n = 3 ± SEM). Statistical analysis was conducted using non-paired Student’s t-test. * = p<0.05; ** = p<0.01; *** = p<0.001; n.s. = non-significant, Prog = progesterone, ctrl = control.

To further explore the mechanisms underlying progesterone cytoprotection, we next examined the physiology of mitochondria, since these organelles constitute one of the main sources of ROS [35][36][37][38]. Interestingly, while O₂ consumption was significantly enhanced during progesterone treatment, ATP levels were reduced (Figure 2B and C). Altogether, this indicates an uncoupling phenotype with diminished oxidative phosphorylation. Accordingly, we observed reduced growth of wild type yeast upon progesterone treatment on a non-fermentable carbon source (glycerol), while no changes were detected on a fermentable carbon source (glucose) (Figure 2D and E). Importantly, this effect was also observed in a mutant strain lacking the heme-binding protein Dap1, which is the sole yeast orthologue of the human progesterone receptor (Figure 2D and E) [39]. Furthermore, we could demonstrate that stress protection by progesterone is respiration-dependent, since progesterone treatment did not confer stress resistance in respiration-deficient rho⁰ cells (Figure 2F). Altogether, it appears that progesterone impacts yeast mitochondrial respiration in a receptor-independent fashion.

FIGURE 2: Progesterone impacts energy metabolism and reduces oxygen stress accumulation in wildtype yeast. Wildtype yeast were treated with 10 µg/ml progesterone and assayed for (A) ROS accumulation via DHE to ethidium turnover, (B) oxygen consumption via respirometry, and (C) ATP production. Growth curves of wildtype as well as ∆dap1 strains, with or without progesterone treatment, on glycerol (respiratory carbon source) (D) and glucose (fermentative carbon source) media (E). ROS accumulation in rho0 yeast cells +/- progesterone (10 µg/ml) treated or untreated with H2O2 or acetate during logarithmic phase (F). All data represent mean values (n = 3-5 ± SEM). Statistical analysis was conducted using non-paired Student t-test (A-C, F) or using a two-way repeated measurement ANOVA and multiple comparison post-hoc Tukey’s test (D, E). * = p<0.05; ** = p<0.01; *** = p<0.001; n.s. = non-significant. ROS = reactive oxygen species, rFU = relative fluorescence units, Prog = progesterone, ctrl = control.

–

Progesterone administration diminishes cytosolic Ca²⁺ concentrations both under physiological as well as under high calcium conditions

Next we investigated progesterone effects on Ca²⁺ homeostasis, knowing that mitochondria are one of the organelles responsible for buffering cytosolic Ca²⁺ under normal conditions [40]. Importantly, TBI, stroke, and even some forms of dementia cause Ca²⁺ accumulation in the cytosol of neurons followed by cell death and neurodegeneration [41]. Thus, we examined the capacity of yeast cells to process Ca²⁺ uptake under the influence of progesterone. Specifically, wild type yeast cell cultures were challenged with 150 mM CaCl₂ and transient concentrations of cytoplasmic Ca²⁺ levels ([Ca²⁺]cyt) / responses were monitored. Progesterone caused a significantly reduced Ca²⁺ uptake capacity alongside with a faster reduction of cytoplasmic Ca²⁺ levels (Figure 3A and B). Of note, basal Ca²⁺ levels before and after the Ca²⁺ pulse were already lowered when cells were treated with progesterone (Figure 3B). However, mitochondrial respiration was not involved in this phenotype, since progesterone treatment continued to affect basic cytosolic Ca²⁺ levels in respiration-deficient rho⁰ cells (Figure 3C and D).

–

FIGURE 3: Cellular Ca2+ homeostasis is modulated by progesterone treatment in yeast. Cells were treated with progesterone (10 µg/ml) and challenged with high doses of Ca2+ (150 mM). Intake of Ca2+ as well as Ca2+-clearance in the cytosol to its basal level were measured in wild type (A,B), a DAP1-deletion strain (C,D), as well as in a rho0 strain, incapable of mitochondrial respiration (E,F). Data are shown as mean values of at least three replicates including the standard error of the mean. Statistical analysis was conducted using non-paired Student’s t-test. * = p<0.05; ** = p<0.01; *** = p<0.001; n.s. = non-significant. Emax = global maximum of the respective ethanol-treated control; rLU = relative luminiscence units, Prog = progesterone, ctrl = control.

To further investigate the observed phenotypes, we tested single-gene deletion mutants of all currently known Ca²⁺ channels/transporters in yeast, including the cytoplasmic membrane transporters Cch1 and Mid 1, the organelle transporters Vcx1, Pmr1, Cod1, Yvc1, and Pmc1 as well as Emc7, an ER protein associated to Ca²⁺ homeostasis. Although Ca²⁺ uptake and clearance was influenced by some of these gene deletion, all mutants continued to exhibit significantly reduced Ca²⁺ uptake when treated with progesterone (compare Supplemental Figure 1A-G to H). Thus, the effects observed in wild type cells could not be reversed by single gene deletions in any of these transporters. Similarly, the effects of progesterone treatment on Ca²⁺ homeostasis/uptake were independent of the mammalian membrane progesterone receptor homolog Dap1 (Figure 3E and F). Taken together, progesterone seems to influence Ca²⁺ homeostasis/uptake in a general manner, independently from known Ca²⁺ transporters and respiration capacity.

DISCUSSION

Here, we establish S. cerevisiae as a model to investigate cytoprotection by progesterone. We observed that progesterone increased stress tolerance of yeast to the well-known PCD inducers H₂O₂ and acetate [14][30][31][32][33][34] as well as under physiological (control) conditions. Interestingly, progesterone treatment led to a mild uncoupling phenotype with higher O₂ consumption (+50%) but lower ATP levels (-50%), arguing for a mitochondrial uncoupling effect. Indeed, growth on the non-fermentable carbon source glycerol was diminished in the presence of progesterone. Notably, mild uncoupling induced by chemical substances (such as dinitrophenol), caloric restriction or ectopic expression of mammalian uncoupling proteins in yeast – S. cerevisiae does not possess any known uncoupling proteins [42] – is known to increase lifespan [43][44][45]. Similarly, in mammalian aging cells, changes in mitochondrial energy metabolism caused by mitochondrial uncoupling seem to improve cellular fitness [46]. Progesterone treatment of human cells has been demonstrated to strongly increase the levels of mRNAs coding for uncoupling proteins [47]. Increased O₂ consumption with decreased ³²P uptake (as a parameter for ATP production) has been reported for isolated rat mitochondria treated with progesterone [48]. Collectively, our data combined with those reported in the literature highlight the possibility to investigate progesterone-mediated effects in the yeast model. The uncoupling aspect of progesterone, in fact, could represent one of the mechanisms of neuroprotection conferred by this steroid. In fact, the stress tolerance of a respiration-deficient rho⁰ strain was not influenced by progesterone treatment.

–

Progesterone had major effects on Ca²⁺ homeostasis and, in particular, on Ca²⁺ susceptibility/uptake. However, we could not identify any single Ca²⁺ channel in yeast that would influence these effects. However, we cannot exclude that yet unidentified Ca²⁺ channels or a combinations of known Ca²⁺ channels mediate these effects [49]. Another possible mode of action of progesterone on Ca²⁺ homeostasis could reside in its direct interaction with biological membranes. Since the chemical structure of progesterone shows four-ring as well as hydrophobic backbone and polar groups at both ends of the molecule, it could directly interact with cellular and mitochondrial membranes [50] and possibly influence their permeability towards inorganic cations (e.g. Ca²⁺, H⁺). This mode of action could connect our observations of mitochondrial uncoupling and modulation of Ca²⁺ homeostasis. Of note, a progesterone-treated rho⁰ strain still showed Ca²⁺ effects but no enhanced stress tolerance, suggesting that altered Ca²⁺ homeostasis may lie upstream of mitochondrial uncoupling. However, these mechanistic hypotheses remain to be empirically tested.

–

Certainly, the putative relevance of the herein described progesterone effects for TBI pathology remains to be explored. In some mammalian cell types, progesterone leads to a significant increase of intracellular Ca²⁺ [51][52], partly by activating protein kinase C [53] and depleting endocannabinoids by activating α/β hydrolase domain-containing protein 2 (ABHD2) [54]. However, in other cell types, progesterone withdrawal leads to an increased level of cytosolic Ca²⁺ [55]. While progesterone was not able to reduce estrogen-induced Ca²⁺ uptake in the rabbit myometrial smooth muscle cells, it increased the accumulation of Ca²⁺ in mitochondria [55]. This suggests that progesterone withdrawal reduces both myometrial cytosolic Ca²⁺ levels as well as the capacity of these cells to accumulate Ca²⁺in different cellular compartments. Similar effects were reported for other types of smooth muscles [56][57] and are believed to be caused by regulation of the inward current through L-type Ca²⁺ channels [56][58]. In neurons, the influence on Ca²⁺ signaling and the following inhibition of excitotoxic neuron death seem to be the neuroprotective mechanism induced by acute administration of progesterone after various neuronal injuries [59][60][61]. Indeed, progesterone might mediate broad neuroprotective effects, not only in the context of TBI but also in other pathologies [62][63].

–

The role of progesterone in the pathological development of TBI has been well described in recent years. It has been shown that progesterone improves the function of the blood-brain-barrier after TBI [64]. Progesterone also increases the level of circulating endothelial cells, which in turn improves neovascularization and vascular remodeling in the brain [65]. Furthermore, progesterone treatment reduces neuroinflammation and oxidative stress [66] as it improves remyelination and functional recovery [63].

–

Interestingly, the intracellular effects exerted by progesterone in our model – reduced intracellular Ca²⁺ levels, uncoupled mitochondria and ROS reduction – were not lost when the sole possible yeast orthologue of the human progesterone receptor was removed from the system. This suggests that progesterone mediates its broad cytoprotective effects through other proteins than steroid receptors or perhaps with cellular membrane lipids. We surmise that yeast constitutes an ideal platform for exploring these effects in further detail.

MATERIALS AND METHODS

Growth conditions

S. cerevisiae strains (Table 1) were inoculated to 5*10⁵ (for growth curve OD₆₀₀ of 0.05) cells in SC medium containing 0.17% yeast nitrogen base (BD Diagnostics; without ammonium sulfate and amino acids), 0.5% (NH₄)₂SO₄ , 30 mg/L of all amino acids (except 80 mg/L histidine and 200 mg/L leucine), 30 mg/L adenine, and 320 mg/L uracil with 2% glucose (SCD) or alternatively with 3% glycerol (SCGly), w/o treatment with progesterone (10 µg/ml; Sigma Aldrich, Catalogue Nr. P0130). Controls were treated with respective solvent (EtOH). Where indicated, stress (acetate or H₂O₂) was inflicted as described previously in mid-log phase (~ 6h of growth, culture density 2-4*10⁶ cells/ml). Due to the inherent reduced respiration-rate of BY4741 strains, TB50a strains were used for respiration-related experiments. DAP1 deletion was carried out by classical homologous recombination [67][68].

Table 1. Strains used in this study. [69]

–

Growth curve

Cells from ONC in SCD media were inoculated to an OD₆₀₀ of 0.05 in SCD media and SCGly media with or without 10 µg/ml progesterone addition. Untreated cultures were supplemented with 0.1% EtOH for solvent control. To obtain growth curves, 300 µl of respective cultures per well were transferred into Honeycomb® plates, and measured with Bioscreen C MBR system (Oy Growth Curves Ab Ltd.) for a period of 48 hours at 28°C, using continuous shaking and OD₆₀₀ measurements every 30 minutes.

–

Oxygen consumption measurement

Oxygen consumption was measured using a FireSting oxygen electrode (Pyro-Science) under constant stirring at a temperature of 28.0 ± 0.2°C in sealed 2 ml bottles. The corresponding cell counts were measured using a CASY Cell Counter, whereas percentage of living cells in the sample were established by flow cytometry with propidium iodide (PI: 100 ng/ml) stained samples. The slope of the oxygen concentration as the function of time in its linear region was calculated and normalized to the number of living cells in the sample.

–

ROS accumulation (DHE) assay

Oxidation of non-fluorescent di-hydroethidium (DHE) to fluorescent ethidium was used to measure ROS accumulation in yeast cells [38]. Approximately 5*10⁶ cells from each sample were collected, washed and incubated with DHE solution (2.5 µg/ml in PBS) for 10 min in the dark. After washing samples were re-suspended in PBS buffer and measured using flow cytometry. The relative mean fluorescence measured for the cell population was used for analysis [70].

–

Boiling ethanol extraction of ATP and ATP measurement

ATP extraction was done with flash-frozen cells by adding 0.5 ml preheated (90°C) BES buffer and incubation at 90°C for 3 minutes. After centrifugation, supernatants were stored at -80°C until the measurement. ATP levels were determined by using the ATP detection kit from Invitrogen in a Luminoskan (Thermo Scientific).

–

Cytosolic Ca²⁺ measurements

[Ca²⁺]cyt were measured using yeast strains carrying the vector pYX212 encoding the bioluminescent protein aequorin under the control of a TPI promoter. For analysis of the cellular response to high doses of external Ca²⁺, an equivalent of 6*10⁶ cells was harvested, resuspended in 200 μl SCD containing 4 μM coelenterazine and incubated for 1 h in the dark. After washing cells were measured in a Luminoskan for 10 s and then challenged with high dose of Ca²⁺ (pump injection of 150 mM Ca²⁺). Kinetics were recorded over 120 s. The luminescence signal was normalized to the OD₆₀₀ of each well and reported in relative luminescence units, normalized to the global maximum value of the ethanol treated control of the respective run for better comparability.

References

1. E. Baulieu, and M. Schumacher, "Progesterone as a neuroactive neurosteroid, with special reference to the effect of progesterone on myelination.", Steroids, 2000. http://www.ncbi.nlm.nih.gov/pubmed/11108866
2. K. Vagnerova, I.P. Koerner, and P.D. Hurn, "Gender and the Injured Brain", Anesthesia & Analgesia, vol. 107, pp. 201-214, 2008. http://dx.doi.org/10.1213/ane.0b013e31817326a5
3. D. Meffre, F. Labombarda, B. Delespierre, A. Chastre, A. De Nicola, D. Stein, M. Schumacher, and R. Guennoun, "Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury", Neuroscience, vol. 231, pp. 111-124, 2013. http://dx.doi.org/10.1016/j.neuroscience.2012.11.039
4. D. Si, H. Wang, Q. Wang, C. Zhang, J. Sun, Z. Wang, Z. Zhang, and Y. Zhang, "Progesterone treatment improves cognitive outcome following experimental traumatic brain injury in rats", Neuroscience Letters, vol. 553, pp. 18-23, 2013. http://dx.doi.org/10.1016/j.neulet.2013.07.052
5. Z. Soltani, M. Khaksari, N. Shahrokhi, G. Mohammadi, B. Mofid, A. Vaziri, and S. Amiresmaili, "Effect of estrogen and/or progesterone administration on traumatic brain injury-caused brain edema: the changes of aquaporin-4 and interleukin-6", Journal of Physiology and Biochemistry, vol. 72, pp. 33-44, 2015. http://dx.doi.org/10.1007/s13105-015-0453-5
6. H.V. Carswell, N.H. Anderson, J.S. Clark, D. Graham, B. Jeffs, A.F. Dominiczak, and I.M. Macrae, "Genetic and gender influences on sensitivity to focal cerebral ischemia in the stroke-prone spontaneously hypertensive rat.", Hypertension (Dallas, Tex. : 1979), 1999. http://www.ncbi.nlm.nih.gov/pubmed/10024327
7. N.J. Alkayed, S.J. Murphy, R.J. Traystman, P.D. Hurn, and V.M. Miller, "Neuroprotective effects of female gonadal steroids in reproductively senescent female rats.", Stroke, 2000. http://www.ncbi.nlm.nih.gov/pubmed/10625733
8. G. Xiao, J. Wei, W. Yan, W. Wang, and Z. Lu, "Improved outcomes from the administration of progesterone for patients with acute severe traumatic brain injury: a randomized controlled trial", Critical Care, vol. 12, 2008. http://dx.doi.org/10.1186/cc6887
9. D.W. Wright, A.L. Kellermann, V.S. Hertzberg, P.L. Clark, M. Frankel, F.C. Goldstein, J.P. Salomone, L.L. Dent, O.A. Harris, D.S. Ander, D.W. Lowery, M.M. Patel, D.D. Denson, A.B. Gordon, M.M. Wald, S. Gupta, S.W. Hoffman, and D.G. Stein, "ProTECT: A Randomized Clinical Trial of Progesterone for Acute Traumatic Brain Injury", Annals of Emergency Medicine, vol. 49, pp. 391-402.e2, 2007. http://dx.doi.org/10.1016/j.annemergmed.2006.07.932
10. B.E. Skolnick, A.I. Maas, R.K. Narayan, R.G. van der Hoop, T. MacAllister, J.D. Ward, N.R. Nelson, and N. Stocchetti, "A Clinical Trial of Progesterone for Severe Traumatic Brain Injury", New England Journal of Medicine, vol. 371, pp. 2467-2476, 2014. http://dx.doi.org/10.1056/NEJMoa1411090
11. C. Lin, H. He, Z. Li, Y. Liu, H. Chao, J. Ji, and N. Liu, "Efficacy of progesterone for moderate to severe traumatic brain injury: a meta-analysis of randomized clinical trials", Scientific Reports, vol. 5, 2015. http://dx.doi.org/10.1038/srep13442
12. Y. Zeng, Y. Zhang, J. Ma, and J. Xu, "Progesterone for Acute Traumatic Brain Injury: A Systematic Review of Randomized Controlled Trials", PLOS ONE, vol. 10, pp. e0140624, 2015. http://dx.doi.org/10.1371/journal.pone.0140624
13. F. Madeo, E. Fröhlich, and K.U. Fröhlich, "A yeast mutant showing diagnostic markers of early and late apoptosis.", The Journal of cell biology, 1997. http://www.ncbi.nlm.nih.gov/pubmed/9348289
14. F. Madeo, E. Fröhlich, M. Ligr, M. Grey, S.J. Sigrist, D.H. Wolf, and K.U. Fröhlich, "Oxygen stress: a regulator of apoptosis in yeast.", The Journal of cell biology, 1999. http://www.ncbi.nlm.nih.gov/pubmed/10330404
15. F. Madeo, E. Herker, C. Maldener, S. Wissing, S. Lächelt, M. Herlan, M. Fehr, K. Lauber, S.J. Sigrist, S. Wesselborg, and K.U. Fröhlich, "A caspase-related protease regulates apoptosis in yeast.", Molecular cell, 2002. http://www.ncbi.nlm.nih.gov/pubmed/11983181
16. E. Herker, H. Jungwirth, K.A. Lehmann, C. Maldener, K. Fröhlich, S. Wissing, S. Büttner, M. Fehr, S. Sigrist, and F. Madeo, "Chronological aging leads to apoptosis in yeast", The Journal of Cell Biology, vol. 164, pp. 501-507, 2004. http://dx.doi.org/10.1083/jcb.200310014
17. S. Wissing, P. Ludovico, E. Herker, S. Büttner, S.M. Engelhardt, T. Decker, A. Link, A. Proksch, F. Rodrigues, M. Corte-Real, K. Fröhlich, J. Manns, C. Candé, S.J. Sigrist, G. Kroemer, and F. Madeo, "An AIF orthologue regulates apoptosis in yeast", The Journal of Cell Biology, vol. 166, pp. 969-974, 2004. http://dx.doi.org/10.1083/jcb.200404138
18. S. Büttner, D. Ruli, F. Vögtle, L. Galluzzi, B. Moitzi, T. Eisenberg, O. Kepp, L. Habernig, D. Carmona-Gutierrez, P. Rockenfeller, P. Laun, M. Breitenbach, C. Khoury, K. Fröhlich, G. Rechberger, C. Meisinger, G. Kroemer, and F. Madeo, "A yeast BH3-only protein mediates the mitochondrial pathway of apoptosis", The EMBO Journal, vol. 30, pp. 2779-2792, 2011. http://dx.doi.org/10.1038/emboj.2011.197
19. L. Galluzzi, O. Kepp, and G. Kroemer, "Mitochondrial regulation of cell death: a phylogenetically conserved control", Microbial Cell, vol. 3, pp. 101-108, 2016. http://dx.doi.org/10.15698/mic2016.03.483
20. S. Büttner, L. Habernig, F. Broeskamp, D. Ruli, F.N. Vögtle, M. Vlachos, F. Macchi, V. Küttner, D. Carmona-Gutierrez, T. Eisenberg, J. Ring, M. Markaki, A.A. Taskin, S. Benke, C. Ruckenstuhl, R. Braun, C. Van den Haute, T. Bammens, A. van der Perren, K. Fröhlich, J. Winderickx, G. Kroemer, V. Baekelandt, N. Tavernarakis, G.G. Kovacs, J. Dengjel, C. Meisinger, S.J. Sigrist, and F. Madeo, "Endonuclease G mediates α-synuclein cytotoxicity during Parkinson's disease", The EMBO Journal, vol. 32, pp. 3041-3054, 2013. http://dx.doi.org/10.1038/emboj.2013.228
21. S. Büttner, F. Broeskamp, C. Sommer, M. Markaki, L. Habernig, A. Alavian-Ghavanini, D. Carmona-Gutierrez, T. Eisenberg, E. Michael, G. Kroemer, N. Tavernarakis, S.J. Sigrist, and F. Madeo, "Spermidine protects against α-synuclein neurotoxicity", Cell Cycle, vol. 13, pp. 3903-3908, 2014. http://dx.doi.org/10.4161/15384101.2014.973309
22. J.J. Heinisch, and R. Brandt, "Signaling pathways and posttranslational modifications of tau in Alzheimer's disease: the humanization of yeast cells", Microbial Cell, vol. 3, pp. 135-146, 2016. http://dx.doi.org/10.15698/mic2016.04.489
23. R. Menezes, S. Tenreiro, D. Macedo, C. Santos, and T. Outeiro, "From the baker to the bedside: yeast models of Parkinson's disease", Microbial Cell, vol. 2, pp. 262-279, 2015. http://dx.doi.org/10.15698/mic2015.08.219
24. T. Amen, and D. Kaganovich, "Yeast screening platform identifies FDA-approved drugs that reduce Aβ oligomerization", Microbial Cell, vol. 3, pp. 97-100, 2016. http://dx.doi.org/10.15698/mic2016.03.482
25. A. Shrestha, and L. Megeney, "Yeast proteinopathy models: a robust tool for deciphering the basis of neurodegeneration", Microbial Cell, vol. 2, pp. 458-465, 2015. http://dx.doi.org/10.15698/mic2015.12.243
26. M. Bond, R. Brown, C. Rallis, J. Bahler, and S. Mole, "A central role for TOR signalling in a yeast model for juvenile CLN3 disease", Microbial Cell, vol. 2, pp. 466-480, 2015. http://dx.doi.org/10.15698/mic2015.12.241
27. D. Carmona-Gutierrez, A.L. Hughes, F. Madeo, and C. Ruckenstuhl, "The crucial impact of lysosomes in aging and longevity", Ageing Research Reviews, vol. 32, pp. 2-12, 2016. http://dx.doi.org/10.1016/j.arr.2016.04.009
28. R. Raghupathi, "Cell death mechanisms following traumatic brain injury.", Brain pathology (Zurich, Switzerland), 2004. http://www.ncbi.nlm.nih.gov/pubmed/15193035
29. G. Cheng, R. Kong, L. Zhang, and J. Zhang, "Mitochondria in traumatic brain injury and mitochondrial‐targeted multipotential therapeutic strategies", British Journal of Pharmacology, vol. 167, pp. 699-719, 2012. http://dx.doi.org/10.1111/j.1476-5381.2012.02025.x
30. P. Ludovico, M.J. Sousa, M.T. Silva, C. Leão, and M. Côrte-Real, "Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid", Microbiology, vol. 147, pp. 2409-2415, 2001. http://dx.doi.org/10.1099/00221287-147-9-2409
31. O. Merkel, D. Asslaber, J. Pinon, A. Egle, and R. Greil, "Interdependent regulation of p53 and miR-34a in chronic lymphocytic leukemia", Cell Cycle, vol. 9, pp. 2836-2840, 2010. http://dx.doi.org/10.4161/cc.9.14.12267
32. T. Eisenberg, D. Carmona-Gutierrez, S. Büttner, N. Tavernarakis, and F. Madeo, "Necrosis in yeast", Apoptosis, vol. 15, pp. 257-268, 2010. http://dx.doi.org/10.1007/s10495-009-0453-4
33. D. Carmona-Gutiérrez, M.A. Bauer, J. Ring, H. Knauer, T. Eisenberg, S. Büttner, C. Ruckenstuhl, A. Reisenbichler, C. Magnes, G.N. Rechberger, R. Birner-Gruenberger, H. Jungwirth, K. Fröhlich, F. Sinner, G. Kroemer, and F. Madeo, "The propeptide of yeast cathepsin D inhibits programmed necrosis", Cell Death & Disease, vol. 2, pp. e161-e161, 2011. http://dx.doi.org/10.1038/cddis.2011.43
34. R.J. Braun, C. Sommer, D. Carmona-Gutierrez, C.M. Khoury, J. Ring, S. Büttner, and F. Madeo, "Neurotoxic 43-kDa TAR DNA-binding Protein (TDP-43) Triggers Mitochondrion-dependent Programmed Cell Death in Yeast", Journal of Biological Chemistry, vol. 286, pp. 19958-19972, 2011. http://dx.doi.org/10.1074/jbc.M110.194852
35. K.M. Holmström, and T. Finkel, "Cellular mechanisms and physiological consequences of redox-dependent signalling", Nature Reviews Molecular Cell Biology, vol. 15, pp. 411-421, 2014. http://dx.doi.org/10.1038/nrm3801
36. R. Braun, C. Sommer, C. Leibiger, R. Gentier, V. Dumit, K. Paduch, T. Eisenberg, L. Habernig, G. Trausinger, C. Magnes, T. Pieber, F. Sinner, J. Dengjel, F. van Leeuwen, G. Kroemer, and F. Madeo, "Accumulation of Basic Amino Acids at Mitochondria Dictates the Cytotoxicity of Aberrant Ubiquitin", Cell Reports, vol. 10, pp. 1557-1571, 2015. http://dx.doi.org/10.1016/j.celrep.2015.02.009
37. R. Braun, C. Sommer, C. Leibiger, R. Gentier, V. Dumit, K. Paduch, T. Eisenberg, L. Habernig, G. Trausinger, C. Magnes, T. Pieber, F. Sinner, J. Dengjel, F. van Leeuwen, G. Kroemer, and F. Madeo, "Modeling non-hereditary mechanisms of Alzheimer disease during apoptosis in yeast", Microbial Cell, vol. 2, pp. 136-138, 2015. http://dx.doi.org/10.15698/mic2015.04.199
38. C. Ruckenstuhl, S. Büttner, D. Carmona-Gutierrez, T. Eisenberg, G. Kroemer, S.J. Sigrist, K. Fröhlich, and F. Madeo, "The Warburg Effect Suppresses Oxidative Stress Induced Apoptosis in a Yeast Model for Cancer", PLoS ONE, vol. 4, pp. e4592, 2009. http://dx.doi.org/10.1371/journal.pone.0004592
39. R.A. Hand, N. Jia, M. Bard, and R.J. Craven, "Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein related to the mammalian membrane-associated progesterone receptor.", Eukaryotic cell, 2003. http://www.ncbi.nlm.nih.gov/pubmed/12684380
40. A. Gregor, M. Kocyłowski, and E. Kostrzewska, "[Evaluation of the diagnostic usefulness of determining porphobilinogen deaminase activity in the erythrocytes in patients with acute intermittent porphyria and in carriers of the gene of this type of porphyria].", Przeglad lekarski, 1986. http://www.ncbi.nlm.nih.gov/pubmed/3575772
41. N. Tubiana, Z. Mishal, F. le Caer, J.M. Seigneurin, Y. Berthoix, P.M. Martin, and Y. Carcassonne, "Quantification of oestradiol binding at the surface of human lymphocytes by flow cytofluorimetry.", British journal of cancer, 1986. http://www.ncbi.nlm.nih.gov/pubmed/3489481
42. D. Roussel, M. Harding, M.J. Runswick, J.E. Walker, and M.D. Brand, "Does any yeast mitochondrial carrier have a native uncoupling protein function?", Journal of bioenergetics and biomembranes, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12171066
43. M.H. Barros, B. Bandy, E.B. Tahara, and A.J. Kowaltowski, "Higher Respiratory Activity Decreases Mitochondrial Reactive Oxygen Release and Increases Life Span in Saccharomyces cerevisiae", Journal of Biological Chemistry, vol. 279, pp. 49883-49888, 2004. http://dx.doi.org/10.1074/jbc.M408918200
44. V.P. Skulachev, "Uncoupling: new approaches to an old problem of bioenergetics", Biochimica et Biophysica Acta (BBA) - Bioenergetics, vol. 1363, pp. 100-124, 1998. http://dx.doi.org/10.1016/S0005-2728(97)00091-1
45. S.A. Mookerjee, A.S. Divakaruni, M. Jastroch, and M.D. Brand, "Mitochondrial uncoupling and lifespan", Mechanisms of Ageing and Development, vol. 131, pp. 463-472, 2010. http://dx.doi.org/10.1016/j.mad.2010.03.010
46. C.E. Amara, E.G. Shankland, S.A. Jubrias, D.J. Marcinek, M.J. Kushmerick, and K.E. Conley, "Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo", Proceedings of the National Academy of Sciences, vol. 104, pp. 1057-1062, 2007. http://dx.doi.org/10.1073/pnas.0610131104
47. A.M. Rodriguez, M. Monjo, P. Roca, and A. Palou, "Opposite actions of testosterone and progesterone on UCP1 mRNA expression in cultured brown adipocytes.", Cellular and molecular life sciences : CMLS, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12475182
48. R. Wade, and H. Jones, " Effect of progesterone on mitochondrial adenosinetriphospatase.", JBC 220;547-551, 1956.
49. W. Liu, "Control of Calcium in Yeast Cells", MS&A, pp. 95-122, 2012. http://dx.doi.org/10.1007/978-88-470-2490-8_5
50. Z.W. Ren, "[Radiofrequency ablation of left-sided atrioventricular accessory tract to treat supraventricular tachycardia].", Zhonghua xin xue guan bing za zhi, 1992. http://www.ncbi.nlm.nih.gov/pubmed/1304487
51. A. Romarowski, C. Sánchez-Cárdenas, H.V. Ramírez-Gómez, L.D.C. Puga Molina, C.L. Treviño, A. Hernández-Cruz, A. Darszon, and M.G. Buffone, "A Specific Transitory Increase in Intracellular Calcium Induced by Progesterone Promotes Acrosomal Exocytosis in Mouse Sperm1", Biology of Reproduction, vol. 94, 2016. http://dx.doi.org/10.1095/biolreprod.115.136085
52. L. Li, C. Xiang, Y. Zhu, and K. Qin, "Modeling of progesterone-induced intracellular calcium signaling in human spermatozoa", Journal of Theoretical Biology, vol. 351, pp. 58-66, 2014. http://dx.doi.org/10.1016/j.jtbi.2014.02.026
53. L. Bonaccorsi, "Progesterone-stimulated intracellular calcium increase in human spermatozoa is protein kinase C-independent", Molecular Human Reproduction, vol. 4, pp. 259-268, 1998. http://dx.doi.org/10.1093/molehr/4.3.259
54. M.R. Miller, N. Mannowetz, A.T. Iavarone, R. Safavi, E.O. Gracheva, J.F. Smith, R.Z. Hill, D.M. Bautista, Y. Kirichok, and P.V. Lishko, "Unconventional endocannabinoid signaling governs sperm activation via the sex hormone progesterone", Science, vol. 352, pp. 555-559, 2016. http://dx.doi.org/10.1126/science.aad6887
55. S. Batra, "Effect of estrogen and progesterone treatment on calcium uptake by the myometrium and smooth muscle of the lower urinary tract.", European journal of pharmacology, 1986. http://www.ncbi.nlm.nih.gov/pubmed/3758176
56. M. Barbagallo, L.J. Dominguez, G. Licata, J. Shan, L. Bing, E. Karpinski, P.K.T. Pang, and L.M. Resnick, "Vascular Effects of Progesterone : Role of Cellular Calcium Regulation.", Hypertension (Dallas, Tex. : 1979), 2001. http://www.ncbi.nlm.nih.gov/pubmed/11208769
57. Y. He, Q. Gao, B. Han, X. Zhu, D. Zhu, J. Tao, J. Chen, and Z. Xu, "Progesterone suppressed vasoconstriction in human umbilical vein via reducing calcium entry", Steroids, vol. 108, pp. 118-125, 2016. http://dx.doi.org/10.1016/j.steroids.2016.02.006
58. Z. Wu, and W. Shen, "Progesterone inhibits L‐type calcium currents in gallbladder smooth muscle cells", Journal of Gastroenterology and Hepatology, vol. 25, pp. 1838-1843, 2010. http://dx.doi.org/10.1111/j.1440-1746.2010.06299.x
59. J.I. Luoma, B.G. Kelley, and P.G. Mermelstein, "Progesterone inhibition of voltage-gated calcium channels is a potential neuroprotective mechanism against excitotoxicity", Steroids, 2011. http://dx.doi.org/10.1016/j.steroids.2011.02.013
60. J.I. Luoma, C.M. Stern, and P.G. Mermelstein, "Progesterone inhibition of neuronal calcium signaling underlies aspects of progesterone-mediated neuroprotection", The Journal of Steroid Biochemistry and Molecular Biology, vol. 131, pp. 30-36, 2012. http://dx.doi.org/10.1016/j.jsbmb.2011.11.002
61. W. Cai, Y. Zhu, K. Furuya, Z. Li, M. Sokabe, and L. Chen, "Two different molecular mechanisms underlying progesterone neuroprotection against ischemic brain damage", Neuropharmacology, vol. 55, pp. 127-138, 2008. http://dx.doi.org/10.1016/j.neuropharm.2008.04.023
62. E. Brotfain, S.E. Gruenbaum, M. Boyko, R. Kutz, A. Zlotnik, and M. Klein, "Neuroprotection by Estrogen and Progesterone in Traumatic Brain Injury and Spinal Cord Injury.", Current neuropharmacology, 2016. http://www.ncbi.nlm.nih.gov/pubmed/26955967
63. J. Wei, and G. Xiao, "The neuroprotective effects of progesterone on traumatic brain injury: current status and future prospects", Acta Pharmacologica Sinica, vol. 34, pp. 1485-1490, 2013. http://dx.doi.org/10.1038/aps.2013.160
64. J.L. Pascual, M.A. Murcy, S. Li, W. Gong, R. Eisenstadt, K. Kumasaka, C. Sims, D.H. Smith, K. Browne, S. Allen, and J. Baren, "Neuroprotective effects of progesterone in traumatic brain injury: blunted in vivo neutrophil activation at the blood-brain barrier", The American Journal of Surgery, vol. 206, pp. 840-846, 2013. http://dx.doi.org/10.1016/j.amjsurg.2013.07.016
65. Z. Li, B. Wang, Z. Kan, B. Zhang, Z. Yang, J. Chen, D. Wang, H. Wei, J. Zhang, and R. Jiang, "Progesterone Increases Circulating Endothelial Progenitor Cells and Induces Neural Regeneration after Traumatic Brain Injury in Aged Rats", Journal of Neurotrauma, vol. 29, pp. 343-353, 2012. http://dx.doi.org/10.1089/neu.2011.1807
66. K.M. Webster, D.K. Wright, M. Sun, B.D. Semple, E. Ozturk, D.G. Stein, T.J. O’Brien, and S.R. Shultz, "Progesterone treatment reduces neuroinflammation, oxidative stress and brain damage and improves long-term outcomes in a rat model of repeated mild traumatic brain injury", Journal of Neuroinflammation, vol. 12, 2015. http://dx.doi.org/10.1186/s12974-015-0457-7
67. U. Gueldener, J. Heinisch, G.J. Koehler, D. Voss, and J.H. Hegemann, "A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.", Nucleic acids research, 2002. http://www.ncbi.nlm.nih.gov/pubmed/11884642
68. U. Güldener, S. Heck, T. Fielder, J. Beinhauer, and J.H. Hegemann, "A new efficient gene disruption cassette for repeated use in budding yeast.", Nucleic acids research, 1996. http://www.ncbi.nlm.nih.gov/pubmed/8692690
69. R. Loewith, E. Jacinto, S. Wullschleger, A. Lorberg, J.L. Crespo, D. Bonenfant, W. Oppliger, P. Jenoe, and M.N. Hall, "Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control.", Molecular cell, 2002. http://www.ncbi.nlm.nih.gov/pubmed/12408816
70. K. Kainz, J. Tadic, A. Zimmermann, T. Pendl, D. Carmona-Gutierrez, C. Ruckenstuhl, T. Eisenberg, and F. Madeo, "Methods to Assess Autophagy and Chronological Aging in Yeast", Methods in Enzymology, pp. 367-394, 2017. http://dx.doi.org/10.1016/bs.mie.2016.09.086