FIGURE 3: Cellular Ca²⁺ homeostasis is modulated by progesterone treatment in yeast. Cells were treated with progesterone (10 µg/ml) and challenged with high doses of Ca²⁺ (150 mM). Intake of Ca²⁺ as well as Ca²⁺-clearance in the cytosol to its basal level were measured in wild type (A,B), a DAP1-deletion strain (C,D), as well as in a rho⁰ strain, incapable of mitochondrial respiration (E,F). Data are shown as mean values of at least three replicates including the standard error of the mean. Statistical analysis was conducted using non-paired Student’s t-test. * = p<0.05; ** = p<0.01; *** = p<0.001; n.s. = non-significant. E_max = global maximum of the respective ethanol-treated control; rLU = relative luminiscence units, Prog = progesterone, ctrl = control.