FIGURE 6: The MPP Mas1 and Mas2 are required for mitochondrial function. (A) Assessment of the conditional mutants for MAS1 and MAS2. MAS1 and MAS2 are depleted using the tetracycline repressible promoter. C. albicans wild-type (WT), tetO-MAS1/mas1D and tetO-MAS2/mas2D cells were grown in the absence (No Dox) or presence (+ Dox) of 20 µg/ml doxycycline for 24 hours and then subcultured in the same conditions for 6 hours; transcript levels of MAS1 and MAS2 were measured and normalised to the ACT1 loading control. (B) Depletion of MAS1 or MAS2 reduces C. albicans growth. The tetO-MAS1/mas1D and tetO-MAS2/mas2D strains were grown in the absence (No Dox) or presence (+ Dox) of 20 µg/ml doxycycline for 24 hours, before being subcultured under the same conditions. Growth was measured for a further 8 hours and OD₆₀₀ was plotted against strains in the absence of depletion. (C) Mas1 is required for oxidative stress survival. The impact of oxidative stress on the tetO-MAS1/mas1Δ and tetO-MAS2/mas2Δ strains and the respective wild type was determined by measurement of CFUs after a 1 hour stress with 5 mM H₂O₂. Percent CFUs was determined rela-tive to untreated cells. Students paired, two-tailed t-test, * p < 0.05. (D) Depletion of either Mas1 or Mas2 leads to res-piratory growth defects. The WT (SN95), tetO-MAS1/mas1 and tetO-MAS2/mas2 strains were grown in the absence (-) or presence (pre-dox) of 20 μg/ml doxycycline in YPD for 24 hours. The cells were then serially diluted (with the lowest dilution of A₆₀₀=1) and plated on YP-based plates containing various carbon sources with (+ Dox) or without (- Dox) doxycycline and incubated at 30^oC or 37^oC for 36 hours.