FIGURE 1: Fission yeast cells are sensitive to FTY720. (A) Quantitative measurements of cell growth in the presence of FTY720. The wt strain (KP178) was grown in liquid YES cultures to an OD₆₆₀ of 0.3 and was treated with FTY720 with the concentrations indicated, and the quantitative measurements of cell growth rates were performed using a microplate reader (SunriseTM series, Tecan, Switzerland). The growth curves of the cells presented are the average for three independent curves. (B) The histograms show the OD₆₆₀ at 10 h, as indicated in Figure 1A. The data were averaged from three independent experiments. Error bars, SD, *P < 0.05, significantly different from FTY720 0 µM using Williams’ test. (C) A serial dilution assay of the wt strain grown in YES medium or YES medium containing the indicated concentrations of FTY720. Cells were incubated for 4 days at 27°C. (D) Work-flow of the screening procedure to uncover genes required for FTY720-resistance. A total of 3,400 strains from Bioneer ver4.0 library were screened. Each of the strains was automatically spotted on YES media containing 0, 10, 20 µM FTY720 and serially diluted and manually streaked and subsequently spotted. 49 strains were found to be repeatedly showing sensitivity on FTY720.