Back to article: Mitochondrial energy metabolism is required for lifespan extension by the spastic paraplegia-associated protein spartin
FIGURE 2: The pro-survival function of spartin depends on functional glycolysis and mitochondrial respiration. (A, C) Survival during chronological aging of WT, Δpfk26 and Δnde1 cells expressing dSp or cells harboring the corresponding ev. Data represent mean values of at least four independent experiments performed at the same time. CFU, colony-forming unit. Error bars represent standard error of mean (SEM). (B, D) Flow cytometry-assisted quantification of oxidative stress levels (conversion of DHE to Eth). (E) Quanti-fication of ATP levels of WT cells expressing dSp or cells harboring the corresponding ev on day 2 of chronological aging. Data represent mean ± SEM of three independent experiments. (F) Cell death during chronological aging of WT and rho0 cells expressing dSp or cells harboring the corresponding ev, quantified by measuring primary and secondary necrotic cells by PI staining, using flow cytometry. Data represent mean ± SEM of four independent experiments.