FIGURE 4: E6AP-dependent degradation of E6-E7. Yeast strain yRA2 was transformed with E6AP, UBCH7 and E6-E7 as described in the legend to Figure 3. Following 1 hour of galactose induction, cells were treated with 50 μg/ml CHX for the indicated time points. Cells were lysed and equivalent amounts of protein extract were analyzed by SDS-PAGE and immunoblotting using the indicated antibodies.