Back to article: Production of poly-β-1,6-N-acetylglucosamine by MatAB is required for hyphal aggregation and hydrophilic surface adhesion by Streptomyces
FIGURE 4: PNAG biosynthesis depends on MatA and MatB. Mycelia from 18 h liquid-grown cultures were stained with mAb F598 (green), showing PNAG-specific staining for the wild-type strain S. lividans 66, while it was absent in mutants lacking matA, matB or the matAB double mutant. Genetic complementation via introduction of pMAT7 expressing matA and matB restored PNAG formation to the matAB double mutant. To visualize the hyphae, the DNA was stained with PI (red). Colocalization of PI and mAb F598 is shown in yellow. Bar: 50 µm.