Back to article: Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae
FIGURE 4: Resumption of growth of S. cerevisiae tps1 mutant transformed with TPS1 from different organism on YN fructose media in microtiter plate. (A) Complementa-tion of sctps1 with TPS1 homologs from yeast species. (B) Complementation of Sctps1 with TPSD1 homologs from P. pastoris, H. polymorpha and the filamentous fungi A. niger and M. grisea. (C) Complementation of sctps1 with TPS1 homologs from bacteria, plants and drosophila. The experimental design was as in Fig.2 except that the yeast cells were inoculated in 250 µL of YN containing fructose at 2% (w/v). For the sake of clarity, standard deviation bars have been withdrawn, but overall the SD was in the range of 10 -15 %.