FIGURE 5: Inhibition of nlrp3 gene expression reduces caspase-1 activation. (A) qPCR for nlrp3 gene expression was performed using RNA extracted from GECs transduced with lentiviruses carrying shRNAs targeting nlrp3 (nlrp3 shRNA) or non-targeting control shRNAs (ctl shRNA). qPCR assays were performed in triplicates, n = 4 independent experiments. Error bars represent ± SD; 2-sided, paired Student's t test, **P < 0.01. (B) Western blot representative image and (C) quantification of caspase-1 of supernatants from lentivirus-transduced GECs. Three independent experiments were performed; a representative blot is shown. Error bars represent ± SD; 2-sided, paired Student's t test, ***P < 0.001.