FIGURE 1: The budding yeast septin collar during the cell cycle. Wild type cells expressing the septin Cdc12 tagged with GFP were imaged at 30°C and Z-stacks were max-projected and pseudocoloured “fire” with Image J. Septins appear at the presumptive bud site in unbudded cells (t=10') and the septin collar splits in late mitosis (t=122', white arrow). DIC: differential interference contrast.