FIGURE 5: Levels of citric acid cycle metabolites in continuously-fed encapsulated yeast differ from planktonic yeast in exponential and stationary phases. Metabolites were extracted from flash-frozen cell pellets grown in batch or in continuous culture as indicated [172] and analyzed by LC-MS using a reversed phase method. Metabolite levels were normalized to the quantity of input cells, and displayed as a log2 fold change centered around the median value for each metabolite.

172. Rosebrock AP, Caudy AA (2017). Metabolite Extraction from Saccharomyces cerevisiae for Liquid Chromatography-Mass Spectrometry. Cold Spring Harb Protoc 2017(9): pdb.prot089086. doi: 10.1101/pdb.prot089086