FIGURE 1: LD biogenesis in the ER. ER-localized triacylglycerol producing enzymes, Lro1, and Dga1 catalyze neutral lipid (NL, indicated in yellow) synthesis from opposite sides of the ER membrane. The NL then accumulate between the two-leaflets of the ER membrane leading to formation of lipid lenses. These NL lenses grow in size to become nascent LDs, which then emerge towards the cytoplasm where they further mature. Fld1 and Nem1 proteins show punctate localization at ER-LD contact sites. The acyltransferase Dga1, and the TAG lipase Tgl3 translocate onto the periphery of mature LDs. Finally, LD-marker proteins such as the perilipin ortholog Pet10 and the sterol biosynthetic enzyme Erg6 decorate the surface of the mature LD.