FIGURE 2: Boxplot of DNA concentration of E. facalis protoplasts. Control protoplasts were not treated by any DNA replication inhibitors. Mitomycin C or novobiocin was added at 24 h of incubation. After that, mitomycin C- and novobiocin-treated protoplasts were used at 48 (24 h-treatment), 72 (48 h-treatment), and 96 h (72 h-treatment) of incubation. Each experiment was performed with 8 replicates.