FIGURE 1: Glucose activates trehalase in a Gpr1 receptor and cAMP-dependent manner. (A) Activation of the PKA target trehalase after addition of 100 mM glucose to glycerol grown, glucose derepressed cells. Strains: wild type (closed circles) and gpr1Δ (open circles). (B) Donor/FRET levels for cAMP determination in vivo upon addition of glucose to glycerol grown cells. Strains: wild type (closed circles) and gpr1Δ (open circles). Mean values of at least three biological replicates, normalised to the value before glucose addition (0 min), are shown with the standard error of the mean. (C) Donor/FRET levels for cAMP determination in vivo upon addition of 100 mM sugar to glycerol grown wild type cells. Sugars: glucose (circles), sucrose (squares) and fructose (triangles).